PCA Ratio

PCA Ratio

Intended use

The PCA Ratio Kit is a clot based assay used in determination of resistance to activated Protein C caused by Factor V Leiden mutation. This APTT based assay creates a clotting time ratio of PCA.APTT/APTT which determines APC resistance.

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Description

PCA Ratio

Main features

  • Activates endogenous Protein C (using Protac®)
  • Excellent discrimination between APC resistant and normal samples
  • Superior result discrimination compared to traditional APC methods
  • Compatible with all instrumentation capable of carrying out APTT assays
  • Negates Lupus and Heparin interference

Kit contents

Component REF Volume Stability
Factor V Depleted Plasma 5546 4 × 2 mL Reconstituted Factor V Depleted Plasma should be discarded after use, or can be frozen at -20°C and thawed once
APTT 5546 2 × 2 mL Reconstituted APTT Reagent is stable for 2 weeks at +2 –+6°C
PCA.APTT 5546 2 × 2 mL Reconstituted PCA.APTT Reagent is stable for 2 weeks at +2 –+6°C
APC Resistant Plasma 5546 1 × 0.5 mL Reconstituted APC Resistant Plasma can be frozen and thawed
Calcium Chloride 0.025M 5546 2 × 8mL Store at +2 –+6°C, and is stable until the expiry date indicated on the label

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